Effects of País grape marc inclusion in high and low forage diets: ruminal fermentation, methane production and volatile fatty acids

The objective of this study was to evaluate the effects inclusion País grape marc (GM) in high (HF) and low forage (LF) ruminant diets on rumen methane (CH4) production fermentation parameters an vitro (batch) culture. Experimental treatments replaced for GM at increasing concentrations (0, 10%, 20% dry matter [DM]) incubation substrates. Gas (GP) CH4 were measured 6, 12 24 h DM disappearance, volatile fatty acids (VFA) ammonia nitrogen (NH3-N) determined h. 10% reduced NH3-N (p < 0.001) by 48% 58% respectively, GM20 BCVFA = 0.049) 21% HF 11% LF. Inclusion percentage gas 0.038), 0.004) yield 0.027), but leads a reduction disappearance (IVDMD) 0.001), total VFA 0.001). In conclusion, batch culture conditions, partial substitution fibre sources has potential reduce with IVDMD.HIGHLIGHTSPaís reduces up regardless level diet.Partial 15%.Partial improve livestock systems sustainability.